δ-OPIOID RECEPTOR ADAPTATION IN NEUROBLASTOMA CELLS

• 作 者: D-M.Chuang M.Belcheva J.Barg J.Rowinski W.G.Clark C.A.Gloeckner A.Ho X-M.Gao C.J.Coscia
• 机 构: Section on Molecular Neurobiology BPB NIMH National Institute of Health Bethesda MD20892 USA,E. A.
• 出 处: 中国药物依赖性通报
• 发表日期: 1993年02期
• 关键词: opioid neuroblastoma naloxone understood simplified disco poorly adaptation expo

The mechanisms underlying tolerance and dependence arising from chronicopioid exposure are poorly understood. However, the development of neuroblastomaand neurohybrid cell cultures has provided a simplified model for the study ofopioid receptor adaptation. Using neuroblastoma NG108-15 cells, we have disco-vered that the up-regulation of σ-opioid receptor sites induced by opioid anta-gonists, naltrexone, naloxone and ICI 174864 is preceded by a transient loss ofreceptor sites. This receptor down-regulation was time- and temperature-

The mechanisms underlying tolerance and dependence arising from chronic opioid exposure are poorly understood. However, the development of neuroblastoma and neurohybrid cell cultures has provided a simplified model for the study of opioid receptor adaptation. Using neuroblastoma NG108-15 cells, we have disco-vered that the up -regulation of σ-opioid receptor sites induced by opioid anta-gonists, naltrexone, naloxone and ICI 174864 is preceded by a transient loss of receptor sites. This receptor down-regulation was time- and temperature-