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目的:探讨60Co灭活的树突状细胞(dendritic cell,DC)诱导的MHC半相合细胞毒性T淋巴细胞(cytotoxicity T lymphocyte,CTL)回输的抗小鼠移植肺癌作用。方法:体外培养CB6F1小鼠来源的DC,流式细胞术检测DC表型,用CB6F1-DC诱导针对C57BL/6小鼠来源的转移性肺癌细胞(Lewis lung cancer,LLC)的细胞毒性T细胞(cytotoxicity T lymphocyte,CTL),经60CO灭活的CTL回输给荷LLC瘤C57BL/6小鼠。细胞毒实验检测CTL细胞的抗肺癌效应,病理检测荷瘤小鼠肝、脾、小肠、皮肤变化,观察移植物抗宿主病(graft-versus-host-disease,GVHD)的发生及肺部肿瘤转移情况,观察荷瘤小鼠的存活时间。结果:流式细胞检测证实培养出成熟DC。DC诱导的CTL灭活后回输治疗LLC肺转移瘤小鼠后,小鼠肺质量显著降低[(0.27±0.06)vs(0.52±0.07)g,P<0.05],平均生存期显著延长[(78.10±16.50)vs(49.30±6.45)d,P<0.05]。荷瘤小鼠脾淋巴细胞对LLC细胞的杀伤活性随着效靶比的增加逐渐增强,最大杀伤率可达(32.7±1.64)%(效靶比100∶1)。病理检测结果显示,治疗组荷瘤小鼠未见明显GVHD。结论:灭活的半相合CTL回输可以诱导机体抗肿瘤反应,降低小鼠移植肺癌转移,未出现明显的GVHD。
Objective: To investigate the anti-mouse transplantation of lung cancer induced by 60Co inactivated dendritic cell (DC) -induced MHC haploidentical cytotoxic T lymphocyte (CTL) transfusion. Methods: CB6F1 mouse-derived DCs were cultured in vitro. The DC phenotypes were detected by flow cytometry. CB6F1-DCs were used to induce cytotoxic T cells against C57BL / 6 mouse-derived lung cancer (LLC) cytotoxicity T lymphocyte (CTL), CTLs inactivated by 60CO were delivered to LLC tumor C57BL / 6 mice. Cytotoxicity assay was used to detect the anti-lung cancer effect of CTL cells. Pathological changes of the liver, spleen, small intestine and skin of tumor-bearing mice were observed. The incidence of graft-versus-host-disease (GVHD) Situation, observed tumor-bearing mice survival time. Results: Flow cytometry confirmed the maturation of DCs. The lung mass of mice with LLC lung metastasis was significantly decreased after DC reinfused CTL inactivation [(0.27 ± 0.06) vs (0.52 ± 0.07) g, P <0.05], and the mean survival time was significantly prolonged [( 78.10 ± 16.50 vs 49.30 ± 6.45 d, P <0.05]. The killing activity of splenic lymphocytes to LLC cells in tumor-bearing mice gradually increased with the increase of the effective target ratio (32.7 ± 1.64)% (target ratio of 100: 1). Pathological examination showed that there was no obvious GVHD in the tumor-bearing mice in the treatment group. CONCLUSION: Inactivated half-matched CTL transfusion can induce the body’s anti-tumor response, reduce the metastasis of lung cancer in mice, and no obvious GVHD.