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目的观察并比较日本血吸虫可溶性成虫抗原(SWA)与可溶性虫卵抗原(SEA)诱导CD4+CD25+Foxp3+调节性T(Treg)细胞水平及其抑制能力的差异。方法分别以PBS、SWA和SEA免疫小鼠,取小鼠脾淋巴细胞,采用流式细胞术检测各组Treg细胞占CD4+T细胞的比例,以及Treg细胞中产IL 10、TGFβ的细胞比例。采用磁珠分选出各组小鼠的Treg细胞后,以3H TdR掺入法检测其抑制靶细胞增殖的能力。结果与SWA相比,SEA可诱导更多的Treg细胞(P<0.05),刺激Treg细胞免疫抑制功能的能力也更强(P<0.05);SWA、SEA免疫小鼠后,SEA刺激Treg细胞产生IL 10、TGFβ的能力更强(P<0.01)。结论SEA较SWA能更好地活化诱导Treg细胞发挥免疫抑制作用。
Objective To observe and compare the differences of CD4 + CD25 + Foxp3 + regulatory T (Treg) cells and their inhibitory ability between soluble adult worm antigen (SWA) and soluble antigen of swine (SEA) of Schistosoma japonicum. Methods Mice were immunized with PBS, SWA and SEA respectively. The spleen lymphocytes of mice were taken out. The proportion of Treg cells to CD4 + T cells and the proportion of cells producing IL 10 and TGFβ in Treg cells were detected by flow cytometry. After the Treg cells of each group were separated by magnetic beads, their ability to inhibit the proliferation of target cells was tested by 3H TdR incorporation. Results Compared with SWA, SEA could induce more Treg cells (P <0.05) and stimulate Treg cells immunosuppressive function (P <0.05). After SWA and SEA immunized mice, SEA stimulated Treg cells to produce IL 10, TGFβ more capable (P <0.01). Conclusion SEA can activate Treg cells more effectively than SWA and play an immunosuppressive role.