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为从分子水平对类杆菌抗性基因和抗性转移进行深入研究，进而寻找出能有效阻断耐药基因传播的措施，并从分子水平上认识医院内感染耐药基因传播与表达的影响因素，作者对临床分离的脆弱类杆菌亚胺培南抗性株的染色体ＤＮＡ进行了分子克隆，获得了１．７ｋｂ的亚胺培南抗性基因片段，而且其重组质粒能在大肠杆菌ＤＨ５中稳定传代和表达。以随机引物法，用地高辛配基标记亚胺培南抗性基因制备探针，对临床分离的４０株类杆菌进行亚胺培南抗性基因检测，结果显示２株亚胺培南抗性株出现杂交信号，３８株亚胺培南敏感株无杂交信号。提示亚胺培南抗性基因探针可用于类杆菌耐药性的分子流行病学调查。
In order to further study the resistance genes and resistance metastasis of Bacteroides from the molecular level, we can find out the effective measures to block the spread of drug resistance genes and understand the influencing factors of the spread and expression of drug resistance genes in the hospital from the molecular level , The authors cloned the chromosomal DNA of clinically isolated fragile Bacillus imipenem-resistant strains and obtained a 1.7kb imipenem-resistant gene fragment, and the recombinant plasmid was stable in E. coli DH5 Passage and expression. Random probe method was used to label imipenem-resistant gene with digoxigenin probe. 40 strains of Bacteroides isolated from clinical isolates were tested for imipenem resistance. The results showed that two strains of imipenem The hybridization signal appeared in the strain, and 38 strains of imipenem sensitive strain did not have the hybridization signal. Prompt imipenem resistance gene probe can be used for molecular epidemiological investigation of drug resistance Bacteroides.