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目的:研究抑制核糖体蛋白L22(ribosomal protein L22,RPL22)基因表达后对ET-1诱导的人肺动脉平滑肌细胞(pul-monary arterial smooth muscle cells,HPASMC)异常增殖的影响。方法:体外原代培养HPASMC,用ET-1诱导其增殖。设计siRNA-RPL22干涉片段,瞬时转染siRNA-RPL22后培养HPASMC,检测其增殖状况。采用Realtime-PCR、Western blot分别检测RPL22mRNA和RPL22蛋白的表达;PCNA、FACScan流式细胞仪检测细胞增殖。结果:HPASMC在转染siRNA-RPL22后,与对照组相比,RPL22 mRNA和RPL22蛋白的表达都显著减少(P<0.05);siRNA-RPL22组HPASMC增殖较对照组显著降低。结论:抑制RPL22表达后,可抑制ET-1诱导的HPASMC增殖,提示RPL22与HPASMC增殖有关,值得进一步深入研究。
Objective: To investigate the effect of ribosomal protein L22 (RPL22) gene expression on the abnormal proliferation of human pulmonary artery smooth muscle cells (HPASMC) induced by ET-1. Methods: HPASMC was cultured in vitro and its proliferation was induced by ET-1. SiRNA-RPL22 interference fragment was designed. After transient transfection of siRNA-RPL22, HPASMC was cultured to detect the proliferation status. Real-time PCR and Western blot were used to detect the expression of RPL22 mRNA and RPL22 protein respectively. Cell proliferation was detected by flow cytometry with PCNA and FACScan. Results: Compared with control group, the expression of RPL22 mRNA and RPL22 protein in HPASMC transfected with siRNA-RPL22 were significantly decreased (P <0.05). The proliferation of HPASMC in siRNA-RPL22 group was significantly lower than that in control group. CONCLUSION: Inhibition of RPL22 expression inhibits the proliferation of HPASMC induced by ET-1, suggesting that RPL22 is associated with the proliferation of HPASMC and deserves further study.