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目的探讨汉防己甲素(tetrandrine,TET)和地塞米松(dexamethasone,DEX)联用对急性百草枯中毒所致肺损伤的拮抗作用。方法大鼠以百草枯(20 mg/kg)腹腔注射染毒后,治疗组经口给予TET(30 mg/kg)或腹腔注射DEX(3 mg/kg)或按上述两种药物的给药时间和剂量同时治疗,未治疗组腹腔注射生理盐水。观察每组动物的中毒表现和死亡结局。分别测定大鼠血浆丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力,并观察肺组织结构改变。结果(1)染毒1 d、3 d时,未治疗组血浆中MDA含量均高于对照组(P<0.05),1 d时血浆中SOD、GSH-Px活力均明显低于对照组(P<0.05);DEX治疗组各时间点血浆中MDA含量和SOD活力与未治疗组相似(P>0.05),1 d时血浆中GSH-Px活力高于未治疗组(P<0.05);TET治疗组除1 d时的GSH-Px活力高于未治疗组外,其余各时点与未治疗组基本一致(P>0.05);联合治疗组1d MDA含量、SOD和GSH-Px活力与未治疗组比较差异有统计学意义(P<0.05);(2)DEX治疗组和联合治疗组的症状与未治疗组相比明显减轻,3 d、7 d存活率均明显高于未治疗组(P<0.05或P<0.01)。TET治疗组大鼠前3 d的中毒症状与未治疗组相似,但程度稍轻,存活率无差异(P>0.05);(3)各治疗组纤维组织增生积分均低于未治疗组(P<0.05或P<0.01),尤以联合治疗组7 d时为著。结论TET和DEX联合治疗可拮抗急性百草枯中毒所致的肺损伤。
Objective To investigate the antagonistic effects of tetrandrine (TET) and dexamethasone (DEX) on lung injury induced by acute paraquat poisoning. Methods After the rats were treated with paraquat (20 mg / kg) intraperitoneally, the rats in the treatment group were given oral TET (30 mg / kg) or intraperitoneal injection of DEX (3 mg / kg) And dose at the same time treatment, untreated group intraperitoneal injection of saline. The poisoning performance and death outcome of each group of animals were observed. Plasma malondialdehyde (MDA) content and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in rats were measured respectively. The lung tissue structure was also observed. Results (1) The content of MDA in the untreated group was significantly higher than that in the control group on day 1 and 3 (P <0.05). At 1 d, the activities of SOD and GSH-Px in plasma were significantly lower than those in the control group <0.05). The plasma levels of MDA and SOD in plasma of DEX treatment group were similar to those of untreated group (P> 0.05), and the activity of GSH-Px in plasma was higher than that of untreated group (P <0.05) The activity of GSH-Px in group 1d was higher than that of untreated group (P> 0.05), and the activity of MDA and SOD and GSH-Px in group 1 were lower than that of untreated group (P <0.05). (2) Compared with the untreated group, the symptoms in the DEX treatment group and the combination treatment group were significantly reduced, and the survival rates on the 3rd and 7th days were significantly higher than those in the untreated group (P < 0.05 or P <0.01). The symptoms of poisoning in the first 3 days in TET treatment group were similar to those in untreated group, but the severity was mild and there was no difference in survival rate (P> 0.05). (3) The proliferation of fibrosis in each treatment group was lower than that in untreated group <0.05 or P <0.01), especially in the combination therapy group at 7 d. Conclusions Combination of TET and DEX can antagonize lung injury caused by acute paraquat poisoning.