论文部分内容阅读
背景:作者前期实验证实国产光敏剂竹红菌乙素(HB)-光动力疗法可引起脉络膜毛细血管的选择性损伤。改变治疗参数进行HB对脉络膜毛细血管1个月内光动力效应的观察,是否会产生新的结果?目的:观察竹红菌乙素-光动力治疗(HB-PDT)对青紫蓝兔脉络膜毛细血管的生物学效应的特点,探讨HB-PDT治疗脉络膜新生血管以及绿光作为PDT治疗光源的研究前景。设计:单一样本研究。单位:解放军总医院激光科。材料:实验于2002-01/2002-11在解放军总医院激光科、病理科及北京理工大学光电工程系完成。光敏剂HB,绿激光器、荧光眼底照相机和透射电子显微镜。方法:使用光纤连接532nm激光器和裂隙灯显微镜,选用青紫兰兔2.5～3.5kg,全麻生效后,耳缘静脉内注射HB1.0mg/kg,532nm光线作为光源激发光敏剂,眼底光斑功率密度300mW/cm2,能量密度30J/cm2,注射药物后立刻照光,光斑直径2000μm,3例,于PDT后1,7,28d观察视网膜,荧光眼底造影、光学显微镜和电子显微镜观察照光部位视网膜和脉络膜的生物学效应。主要观察指标:直接眼底观察视网膜的非选择性损伤,荧光眼底造影观察脉络膜血管系统的闭塞情况,光学显微镜初步观察视网膜非选择性损伤的部位和程度和脉络膜的血管结构改变,电子显微镜观察眼底组织改变的超微结构的变化。结果:PDT后1d,照光区
Background: The author’s previous experiments confirmed that the domestic photosensitizer Hypocrellin (HB) -Photodynamic therapy can cause selective damage to choroidal capillaries. Change the treatment parameters HB observed on the photodynamic effect of choroidal capillaries 1 months, will produce new results? Objective: To observe the hypocrellin B-photodynamic therapy (HB-PDT) on the choriocapillaris capillaries To explore the biological characteristics of HB-PDT treatment of choroidal neovascularization and green light as PDT treatment of light source research prospects. Design: Single Sample Study. Unit: PLA General Hospital Laser Division. MATERIALS: Experiments were performed at the Department of Optics and Electronics, PLA General Hospital, Department of Pathology and Beijing Institute of Technology from January 2002 to November 2002. Photosensitizer HB, a green laser, a fluorescence fundus camera and a transmission electron microscope. Methods: Using optical fiber to connect 532nm laser and slit lamp microscope, 2.5 ~ 3.5kg Qingzhu rabbits were chosen. After the effect of general anesthesia, the rabbits were injected with HB1.0mg / kg, 532nm light as light source to induce photosensitizer. The power density of fundus spot was 300mW / cm2, energy density 30J / cm2, immediately after injection of the drug light, spot diameter 2000μm, 3 cases, in the PDT after 1, 7, 28d observe the retina, fluorescein fundus angiography, optical microscope and electron microscope retina and choroid biological Learning effect. MAIN OUTCOME MEASURES: non-selective retinal damage was observed by direct fundus, occlusion of choroidal vasculature was observed by fluorescence fundus. The location and extent of non-selective retinal damage and changes of choroidal vascular structure were observed by optical microscope. Changes in ultrastructural changes. Results: 1 d after PDT, light area