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众所周知,大豆脂氧合酶(LOX)活性与生长发育密切相关。业已证实,PEG(聚乙二醇)处理大豆种子可促进其下胚轴中蛋白质的合成。本实验结果表明:PEG处理大豆种子后,其下胚轴中的脂氧合酶活性与未经PEG处理的对照相比显著降低。而蛋白质的含量却高于对照。因此推测,下胚轴中引起酶促脂质过氧化作用的主要酶—脂氧合酶活性的降低,与蛋白质总含量的升高密切相关。 PEG高渗溶液具有引发种子的效果,一方面是由于PEG处理可以改善种子的膜透性使损伤得到恢复,因而提高种子活力。另一方面是由于PEG处理种子会引起ATP的迅速合成与消耗,最终使新陈代谢作用增强的缘故。对植物体内LOX活性与蛋白质含量间关系的研究报道不多,且争论颇大。本文在PEG处理种子的前提下,首次探讨了大豆下胚轴脂氧合酶活性与蛋白质含量之间的关系,为LOX生理功能和PEG引发种子机制的进一步研究提供了依据。
As we all know, soybean lipoxygenase (LOX) activity and growth and development are closely related. It has been demonstrated that PEG (polyethylene glycol) treatment of soybean seeds can promote the synthesis of proteins in their hypocotyls. The results of this experiment showed that the lipoxygenase activity in hypocotyls of PEG treated soybean decreased significantly compared with the control without PEG treatment. The protein content is higher than the control. Therefore, we hypothesize that the decrease of the activity of lipoxygenase, which is the major enzyme in the hypocotyls, induces lipid peroxidation, is closely related to the increase of the total protein content. The PEG hypertonic solution has seed-inducing effect, on the one hand, as the PEG treatment improves the membrane permeability of the seed and restores the damage, thus improving seed vigor. On the other hand, PEG treatment of seeds will lead to the rapid synthesis and consumption of ATP, and ultimately enhance the metabolism of sake. There are few reports on the relationship between LOX activity and protein content in plants, and there is much controversy. In this paper, the relationship between lipoxygenase activity and protein content of soybean hypocotyls was discussed for the first time on the premise of seed treatment with PEG, which provided the basis for further study on the physiological functions of LOX and PEG-induced seed mechanism.