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为了研究煤尘对石英的细胞毒效应有无影响,作者将豚鼠的肺泡与腹膜巨噬细胞分别培养于未加与加有5%豚鼠血清的培养基内,并以颗粒<5微米的标准石英(DQ_(12)),与同样大小的(无烟)煤尘,先经121℃高压蒸汽灭菌后,分别及合钟放入细胞培养基中,于37℃温育后进行观察。实验结果表明,石英对肺泡巨噬细胞的损害作用与其作用时间有关。在加入100微克石英的不含血清的培养基中,5小时后已有56%的细胞因丧失其活性而染上了赤藓红B。至第20小
In order to investigate whether coal dust has an effect on the cytotoxic effect of quartz, the authors cultured the alveoli and peritoneal macrophages of guinea pigs in culture medium without addition of 5% guinea pig serum and cultured with standard quartz (DQ_ (12)) and the same size (smoke-free) coal dust were sterilized by autoclaving at 121 ° C, respectively, and then placed in the cell culture medium for a total of eight hours and incubated at 37 ° C for observation. The experimental results show that the damage effect of quartz on alveolar macrophages is related to its action time. In a serum-free medium supplemented with 100 micrograms of quartz, 56% of the cells had lost their activity and erythrosine B had been colored after 5 hours. To the 20th small