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目的探讨低氧诱导因子鄄1α在脑胶质瘤中的表达及其意义。方法60例脑胶质瘤标本中Ⅰ级11例(毛细胞型星形细胞瘤8例、脉络丛乳头状瘤2例、黏液乳头状型室管膜瘤1例);Ⅱ级20例(弥漫型星形细胞瘤11例、少突胶质细胞瘤5例、室管膜瘤2例、多形性黄色瘤型星形细胞瘤2例);Ⅲ级21例(间变性星形细胞瘤12例、间变性少突胶质细胞瘤6例、间变性室管膜瘤3例);Ⅳ级8例(均为胶质母细胞瘤)。采用免疫组织化学方法检测胶质瘤标本中低氧诱导因子鄄1α的表达变化,并与胶质瘤体积及患者年龄、性别进行统计学分析。结果(1)胶质瘤组织低氧诱导因子鄄1α表达呈阳性反应,主要位于细胞质和(或)细胞核,具有明显的异质性;肿瘤浸润边缘部的肿瘤细胞表达明显增强;而阴性对照标本和10例对照脑组织标本则无表达。胶质瘤组织中低氧诱导因子鄄1α阳性表达率为71.67%(43/60),其中Ⅰ级为27.27%(3/11),Ⅱ级70.00%(14/20),Ⅲ级85.71%(18/21),Ⅳ级100%(8/8)。高级别胶质瘤者低氧诱导因子鄄1α阳性表达率明显高于低级别者,不同级别间差异有高度统计学意义(χ2=15.907,P<0.01);表达强度与病理级别间呈高度正相关(rs=0.480,P<0.01)。(2)低氧诱导因子鄄1α表达与患者年龄、性别及原发肿瘤体积的大小等均无相关性(均P>0.05)。结论脑胶质瘤低氧诱导因子鄄1α的表达强弱与肿瘤病理分级相关。
Objective To investigate the expression of hypoxia inducible factor-1α in glioma and its significance. Methods Sixty Ⅰ grade glioma samples were obtained from 11 patients (8 with astrocytoma of hair cell type, 2 with choroid plexus papilloma, 1 with mucinous papillary ependymoma) and 20 with grade Ⅱ (diffuse 11 cases of astrocytoma, 5 cases of oligodendroglioma, 2 cases of ependymoma and 2 cases of pleomorphic astrocytoma of pleomorphic xanthoma; Ⅲ grade 21 cases (anaplastic astrocytoma 12 Cases, anaplastic oligodendroglioma in 6 cases, anaplastic ependymoma in 3 cases); Ⅳ grade in 8 cases (all glioblastoma). Immunohistochemistry was used to detect the expression of hypoxia-inducible factor-1α in glioma specimens, and statistical analysis was made with glioma volume and patient’s age and gender. Results (1) Hypoxia-inducible factor-1alpha expression in glioma tissue was positive, mainly located in the cytoplasm and / or nucleus, with obvious heterogeneity. The expression of tumor cells in the marginal part of tumor infiltration was significantly increased. And 10 cases of control brain tissue specimens were not expressed. The positive rate of hypoxia inducible factor-1α in glioma tissues was 71.67% (43/60), among which, grade Ⅰ was 27.27% (3/11), grade Ⅱ 70.00% (14/20), grade Ⅲ 85.71% 18/21), grade IV 100% (8/8). The positive expression rates of HIF-1α in high-grade gliomas were significantly higher than those in lower grades (χ2 = 15.907, P <0.01). The positive expression rates of HIF-1α in high grade gliomas were highly positive (Rs = 0.480, P <0.01). (2) There was no correlation between the expression of HIF-1α and the patient’s age, sex and the size of primary tumor (all P> 0.05). Conclusion The expression of hypoxia inducible factor-1α in glioma is correlated with tumor grade.