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萌芽素和类萌芽素蛋白在不同植物的各个生长阶段和胁迫相关过程中起到不同的作用。本研究首次从刚毛柽柳cDNA文库中获得类萌芽素蛋白全长基因ThGLP,该基因编码225个氨基酸,含有植物萌芽素和类萌芽素蛋白的功能序列。通过进化树分析发现该基因屑于真正萌芽素亚家族。利用实时定量PCR方法研究了该基因在PEG、NaCl、低温、CdCl2和ABA胁迫下不同时间的表达模式。结果显示PEG、NaCl、低温、CdCl2和ABA处理均能诱导ThGLP基因在柽柳的根和叶中的表达。结果表明ThGLP在柽柳根和叶中表达,参与非生物胁迫应答并由ABA依赖的信号传导途径调控。
Germin and sprouting hormone proteins play different roles in different stages of growth and stress-related processes in different plants. In this study, we obtained the full-length germ-like protein ThGLP gene from Glyptosternicaria laevigata cDNA library for the first time. The gene encodes 225 amino acids and contains the functional sequences of plant buds and sprouts. Phylogenetic analysis revealed that the gene was found in the true budding subfamily. The expression pattern of this gene at different time under PEG, NaCl, low temperature, CdCl2 and ABA stress was studied by real-time PCR. The results showed that the treatment of PEG, NaCl, low temperature, CdCl2 and ABA could induce the expression of ThGLP in the roots and leaves of Tamarix. The results show that ThGLP is expressed in the roots and leaves of tamarisk, is involved in abiotic stress responses and is regulated by ABA-dependent signaling pathways.