论文部分内容阅读
目的探讨髓鞘碱性蛋白(myelin basic protein,MBP)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)在实验性胰性脑病大鼠模型血清及脑脊液中水平的变化,分析各因子与胰性脑病发生发展的关系,为临床胰性脑病的诊治提供实验依据。方法选取40只SD大鼠随机分为假手术组(SO组,n=10)和胰性脑病组(PE组,n=30),经十二指肠乳头逆行胰胆管穿刺注射生理盐水(SO组)或5%牛磺胆酸钠(PE组)诱导建立大鼠胰性脑病模型。SO组大鼠于手术后1 d全部处死,PE组分别于造模后1 d、3 d及7 d各处死10只,取各组大鼠的脑组织和胰腺组织,观察其病理学改变,并观察脑微血管内白细胞聚集及附壁现象;检测脑组织含水量,以及血清和脑脊液中MBP、TNF-α和TL-6的含量。结果 PE组大鼠造模后随时间延长,脑神经细胞水肿及神经纤维脱髓鞘改变明显;血清及脑脊液中MBP、TNF-α和IL-6的含量在造模后1 d、3 d及7 d均明显高于SO组(P<0.05),且随时间的延长逐渐升高,但经两两比较,显示上述3项指标的变化趋势有所不同。结论 MBP、TNF-α和IL-6对胰性脑病脑损害的发生和发展发挥了协同作用;通过检测血液及脑脊液中MBP、TNF-α及IL-6的含量可对胰性脑病病情进行诊断和评估。
Objective To investigate the levels of myelin basic protein (MBP), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in serum and cerebrospinal fluid of experimental rat model of encephalopathy The relationship between each factor and the occurrence and development of pancreatic encephalopathy was analyzed to provide experimental evidence for the diagnosis and treatment of clinical pancreatic encephalopathy. Methods Forty Sprague-Dawley rats were randomly divided into sham operation group (SO group, n = 10) and encephalopathy group (PE group, n = 30) Group) or 5% sodium taurocholate (PE group) induced rat pancreatic encephalopathy model. The rats in SO group were all sacrificed on the first day after operation. In PE group, 10 rats were sacrificed on the 1st, 3rd, and 7th day respectively. The brain tissues and pancreatic tissues of the rats in each group were observed, Cerebral microvascular endothelial cell adhesion and adhesion were observed. The water content of brain tissue and the contents of MBP, TNF-α and TL-6 in serum and cerebrospinal fluid were detected. Results After the model was established in PE group, the edema of brain cells and demyelination of nerve fibers changed obviously with the passage of time. The levels of MBP, TNF-α and IL-6 in serum and cerebrospinal fluid of rats in 1 h, 3 d and 7 d were significantly higher than the SO group (P <0.05), and with the extension of time gradually increased, but after a couple of comparison, shows the trend of these three indicators of the change. Conclusions MBP, TNF-α and IL-6 play a synergistic role in the pathogenesis and development of pancreatic encephalopathy. By detecting the levels of MBP, TNF-α and IL-6 in blood and cerebrospinal fluid, the diagnosis of pancreatic encephalopathy can be diagnosed And evaluation.