液相色谱-电喷雾电离三重四级杆质谱测定畜禽肝脏中维吉尼亚霉素M1的残留量

• 作 者: 魏云计 冯民 刘艳 黄明 王艳 殷耀 周光宏 吴斌
• 机 构: 南京农业大学食品科技学院/肉品加工与质量控制教育部重点实验室,淮安出入境检验检疫局,江苏出入境检验检疫局食品实验室,
• 出 处: 分析试验室
• 发表日期: 2012年04期
• 关键词: 电喷雾电离 M1 维吉尼亚 四级 固相萃取 维吉尼亚霉素M1 分散型 色谱 乙酸乙酯提取 质谱

建立了动物肝脏组织中维吉尼亚霉素M1残留的液相色谱-电喷雾电离三重四级杆质谱(LC-ESI-MS/MS)的测定方法。样品经0.2 mol/L NH4H2PO4和乙酸乙酯提取,分散型固相萃取PSA填料净化,采用正离子扫描多反应监测(MRM)模式下液质联用仪进行定性和定量分析。该方法省去耗时的固相萃取过程,回收率在67.2%～90.3%之间,RSD<10.0%;检测限为0.5μg/kg,定量限为1.0μg/kg。

A liquid chromatography-electrospray ionization triple quadrupole mass spectrometry (LC-ESI-MS / MS) method was developed for the determination of virginiamycin M1 residues in animal liver tissues. The samples were extracted with 0.2 mol / L NH4H2PO4 and ethyl acetate, and dispersed solid phase extraction (PSA) plugs were purified. The samples were qualitatively and quantitatively analyzed by LC-MS in positive ion scanning multiple reaction monitoring (MRM) mode. The method eliminates the time-consuming solid-phase extraction process. The recoveries ranged from 67.2% to 90.3% with RSD <10.0%. The detection limit was 0.5μg / kg and the limit of quantification was 1.0μg / kg.