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在生物医学和临床医学领域,许多疾病的诊断和治疗依赖于细胞形态的识别。不同细胞具有不同的形态,这些形态的不同将导致生物组织中光传播特征的变化,更重要的是这将影响细胞的光散射特性。目前,动态光散射理论是动态识别细胞尺寸和形状的最佳方式。细胞主要由细胞质、细胞核和线粒体组成,因此,分析它们的光散射特性对于光学诊断和治疗具有非常重要的意义。设计实验获取了癌细胞和聚苯乙烯球的光散射特性,并利用时域有限差分法建立细胞质模型进行细胞光散射特性仿真。从肺癌细胞的光散射结果可以看出,线粒体对前向散射(0°~20°)和后向散射(160°~180°)贡献最大,细胞核对侧向散射(80°~100°)贡献最大,细胞质对各个角度贡献均等。仿真结果和实验结果基本一致。
In the field of biomedicine and clinical medicine, the diagnosis and treatment of many diseases depend on the identification of cell morphology. Different cells have different morphologies. The difference of these morphologies will lead to the variation of light propagation characteristics in biological tissues. More importantly, this will affect the light scattering properties of cells. At present, the dynamic light scattering theory is the best way to dynamically identify cell size and shape. Cells are mainly composed of cytoplasm, nucleus and mitochondria, therefore, analyzing their light scattering properties is of great significance for optical diagnosis and treatment. The light scattering properties of cancer cells and polystyrene spheres were obtained through the design experiments. The time-domain finite difference method was used to establish the cytoplasmic model to simulate the light scattering characteristics of cells. From the light scattering results of lung cancer cells, it can be seen that mitochondria contributed the most to forward scatter (0 ° ~ 20 °) and backscatter (160 ° ~ 180 °), and nucleus contributed to lateral scatter (80 ° ~ 100 °) The largest cytoplasm contributes equally to all angles. Simulation results and experimental results are basically the same.