论文部分内容阅读
目的探讨人参皂苷Rg1对血管紧张素Ⅱ(AngⅡ)诱导人脐静脉内皮细胞(HUVECs)凋亡的逆转作用。方法体外培养HUVECs,采用台盼蓝染色方法筛选人参皂苷Rg1最适浓度,采用琼脂糖凝胶电泳和末端脱氧核糖核苷酸转移酶介导的缺口末端标记法(TUNEL)染色检测细胞凋亡情况。结果台盼蓝染色显示人参皂苷Rg1的最适浓度为40μg/mL。琼脂糖凝胶电泳显示,对照组和Rg1组未见凋亡条带,AngⅡ组可见清楚的细胞凋亡的“梯状”条带,而AngⅡ+Rg1组可见不明显的细胞凋亡的“梯状”DNA断裂条带。TUNEL染色显示,与对照组和Rg1组相比,AngⅡ组细胞凋亡数量增加,差异有统计学意义(P<0.01);与AngⅡ组相比,AngⅡ+Rg1组细胞凋亡数量减少,差异有统计学意义(P<0.01),凋亡百分比由38.667%下降到10.667%,但仍高于对照组和Rg1组(P<0.01)。结论 40μg/mL人参皂苷Rg1可一定程度逆转AngⅡ诱导的HUVECs凋亡。
Objective To investigate the reversal effect of ginsenoside Rg1 on the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by angiotensin Ⅱ (AngⅡ). Methods HUVECs were cultured in vitro. The optimal concentration of ginsenoside Rg1 was screened by Trypan blue staining. The apoptosis of cells was detected by agarose gel electrophoresis and terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL) staining . Results Trypan blue staining showed that the optimal concentration of ginsenoside Rg1 was 40μg / mL. Agarose gel electrophoresis showed no apoptosis bands in the control group and the Rg1 group, and a clear “ladder” band of apoptosis was observed in the group of AngⅡ, but no obvious apoptosis was observed in the group of AngⅡ + Rg1 “Ladder ” DNA rupture band. TUNEL staining showed that compared with the control group and the Rg1 group, the number of apoptotic cells in the AngⅡ group increased (P <0.01), and the number of apoptotic cells in the AngⅡ + Rg1 group decreased compared with that in the AngⅡ group The percentage of apoptosis decreased from 38.667% to 10.667%, but still higher than that of the control and Rg1 groups (P <0.01). Conclusion 40μg / mL ginsenoside Rg1 can reverse Ang Ⅱ-induced apoptosis of HUVECs to some extent.