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Agricultural water allocation system based on priority rights has caused regional conJtiets and downstream ecological degradation.It is the urgent need to introduce the concept of the initial water rights and establish benefits compensation mechanism to resolve such problems.This paper takes the Shivang River basin as an example to calculate the opportunity cost of 0.97×108m3 of agricultural water encroached by the middle reach based on initial water right allocation system under which water is allocated in accordance with the ratio between agricultural population of two different regions concerning the downstream ecological reconstruction needs with Bio-economic model (BEM).The results suggest that the total economic loss of Minqin County for ecological econstruction amounts to 2.57×108 yuan,of which 1.68×108 yuan is ecological compensation,representing the economic loss Minqin suffered for ecological reconstruction which should burden beneficial groups of eeological reconstruction and 0.89×108 yuan is the economic loss Minqin suffered due to Liangzhou's encroachment behavior which should be compensated by Liangzhou.