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目的:探讨大肠杆菌麦芽糖结合蛋白(MBP)在小鼠肝癌H22荷瘤模型中的抗肿瘤作用及可能机制。方法:将12只6~8周龄的BALB/c小鼠皮下注射2×10~6个H22细胞,建立荷瘤模型,荷瘤后第2天将小鼠分为PBS组和MBP组,每组6只,并通过皮下瘤旁注射给药的方式进行干预,期间监测肿瘤体积和小鼠体重变化,测量肿瘤和各脏器质量,ELISA法检测脾细胞分泌IL-12和IFN-γ的水平,Real-time PCR检测肿瘤组织中IFN-γ和IL-17A mRNA的相对表达量。结果:MBP能抑制肿瘤的生长(P<0.05)。与PBS组相比,MBP组小鼠脾细胞中IL-12、IFN-γ分泌量上升,肿瘤组织中IFN-γmRNA的表达量上升,IL-17A mRNA的表达量下降(P<0.05)。结论:MBP能够有效抑制肝癌H22肿瘤生长,其作用可能是通过激活Th1型免疫反应和抑制Th17型免疫反应。
Objective: To investigate the antitumor effect of E. coli maltose binding protein (MBP) in H22 tumor-bearing mice and its possible mechanism. Methods: Twelve BALB / c mice aged 6-8 weeks were injected subcutaneously with 2 × 10 ~ 6 H22 cells to establish a tumor-bearing model. On the second day after tumor implantation, mice were divided into PBS group and MBP group. Group 6 were injected subcutaneously by the way of intervention, during the monitoring of tumor volume and body weight changes in mice, measuring the quality of the tumor and various organs, ELISA method to detect the level of IL-12 and IFN-γ secreted by the spleen cells Real-time PCR was used to detect the relative expression of IFN-γ and IL-17A mRNA in tumor tissue. Results: MBP inhibited tumor growth (P <0.05). Compared with PBS group, the secretion of IL-12 and IFN-γ in the spleen cells of MBP group increased, the expression of IFN-γmRNA in tumor tissue increased and the expression of IL-17A mRNA decreased (P <0.05). CONCLUSION: MBP can effectively inhibit the growth of H22 tumor in hepatocellular carcinoma, and its effect may be through activating Th1-type immune response and inhibiting Th17-type immune response.