论文部分内容阅读
【目的】探讨血管紧张素Ⅱ受体抑制剂缬沙坦对心肌缺血大鼠缝隙连接蛋白Cx43表达的影响。【方法】取60只SD大鼠，随机分为空白对照组（A组）、假手术组（B组）、模型组（C组）和缬沙坦组（D组）。空白对照组未做处理， B组进行切口和缝合，不进行左冠状动脉结扎操作；C组进行左冠状动脉结扎操作，建立心肌缺血模型；D组建模前给予缬沙坦灌胃预处理7 d，7 d后建立心肌缺血模型，模型建立1 h后处死大鼠。比较四组心肌组织病理学改变、心肌组织Cx43蛋白和基因的表达水平。【结果】D组心肌缺血病理改变较C组减轻;C组心肌Cx43蛋白和基因表达水平显著低于A组和B组（P<0.05），D组Cx43蛋白和基因表达水平显著高于C组（P<0.05）。【结论】缬沙坦预处理可减轻心肌缺血诱导的Cx43降低，减轻心肌缺血组织病理改变，对心肌缺血大鼠具有保护作用。|%【Objective】To investigate the effect of angiotensin Ⅱ receptor inhibitor valsartan on the expression of gap connexin Cx43 in rats with myocardial ischemia. 【Methods】Sixty SD rats were randomly divided into blank control group (group A), sham operation group (group B), model group (group C) and valsartan group (group D). The Group D was pretreated with valsartan, and the groups of A, B and C were given equal amount of saline, and 7d was used continuously. The model of myocardial ischemia was established in the group C and the group D, and the rat was killed after the model was set up by 1h. The histopathological changes, expression of Cx43 protein and the gene in myocardium of the 4 groups were observed. 【Results】The pathological changes of myocardial ischemia in the group D were less than that in the group C. The expression level of Cx43 protein and gene in the C group was significantly lower than that in the group A and the group B (P<0.05), and the expression level of Cx43 protein and gene in the group D was significantly higher than that inthe C group (P<0.05). 【Conclusion】Valsartan preconditioning can reduce the decrease of Cx43 induced by myocardial ischemia and alleviate the pathological changes of myocardial ischemia tissue, and have protective effect on myocardial ischemia rats.