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目的探讨ATP-生物荧光法肿瘤化疗药敏试验在鼻咽癌化疗药物筛选中的应用价值。方法采用ATP-生物荧光法和MTT法分别对人鼻咽低分化鳞癌细胞株(CNE-2)进行12种化疗药物的肿瘤化疗药敏试验。结果 ATP-TCA法与MTT法所求得作用于CNE-2细胞的IC_(50)比较,其差异在统计学上无意义(P>0.05)。ATP-TCA法检测CNE-2对12种化疗药物的敏感性依次为:顺铂(DDP)、丝裂霉素(MMC)、紫杉醇(TXL)、长春瑞宾(NVB)、卡铂(KB)、羟基喜树碱(HCT)、五氟尿嘧啶(5-FU)、长春新碱(VCR)、更生霉素(ACTD)、鬼臼碱(Vp-16)、吡柔比星(THP)、表阿霉素(EPI)。结论认为ATP肿瘤体外药敏试验(ATP-TCA)应用于鼻咽癌化疗药物的筛选,具有敏感、快速、与临床相关性较好的特点,是目前最有发展前途的体外药敏试验方法;DDP和TXL合用可提高对CNE-2肿瘤细胞的抑制率。
Objective To investigate the value of ATP-biofluorescence tumor chemosensitivity test in the screening of chemotherapy drugs for nasopharyngeal carcinoma. Methods ATP-biofluorescence assay and MTT assay were used to evaluate the chemosensitivity of 12 chemotherapeutic drugs to human nasopharyngeal carcinoma cell line (CNE-2). Results The difference of IC 50 between CNE-2 cells and ATP-TCA assay was statistically insignificant (P> 0.05). The sensitivities of CNE-2 to 12 chemotherapeutic drugs by ATP-TCA were as follows: cisplatin (DDP), mitomycin (MMC), paclitaxel (TXL), vinorelbine (NVB) (HCT), 5-FU, VCR, ACTD, Vp-16, THP, Mycophenolate (EPI). Conclusions ATP-TCA is the most promising drug susceptibility testing method in vitro for the screening of chemotherapeutic drugs for nasopharyngeal carcinoma. It is sensitive, rapid and clinically relevant. The combination of DDP and TXL increases the inhibition of CNE-2 tumor cells.