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通过共培养方法,以细胞密度为主要测定指标,研究了小珊瑚藻对赤潮异弯藻的克生效应及其对UV-B辐射增强的响应.结果表明:小珊瑚藻的新鲜组织和水溶性抽提液对赤潮异弯藻的生长表现出显著的抑制作用(P<0.05),说明小珊瑚藻对赤潮异弯藻具有克生效应;而小珊瑚藻干粉末和培养水过滤液对赤潮异弯藻生长则无明显影响(P>0.05).将小珊瑚藻用不同剂量的UV-B辐射预处理后,再与赤潮异弯藻共培养,除培养水过滤液外,其新鲜组织、干粉末和水溶性抽提液对赤潮异弯藻生长的作用效果均有所改变,高剂量(3.0J.m-2)UV-B辐射使小珊瑚藻对赤潮异弯藻的抑制作用减弱(P<0.05),而低剂量(0.9J.m-2)UV-B辐射则使抑制作用增强(P<0.05).
The co-culture method was used to determine the cell density as the main index to study the effects of Coral Alga on the growth of Heterosigma akashiwo and its response to UV-B radiation.The results showed that fresh tissue and water-soluble The extract had a significant inhibitory effect on the growth of Heterosigma akashiwo (P <0.05), indicating that Coral Reef had a synergistic effect on Heterosigma akashiwo; (P> 0.05) .After pretreated with different doses of UV-B radiation, Coral Reefs were co-cultured with Heterosigma akashiwo, except for the culture filtrate, the fresh tissue and dry The effect of powder and water-soluble extract on the growth of Heterosigma akashiwo had been changed. High-dose (3.0Jm-2) UV-B radiation reduced the inhibitory effect of C.albicans on Heterosigma akashiwo (P <0.05 ), While UV-B radiation at low dose (0.9Jm-2) enhanced the inhibitory effect (P <0.05).