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目的评定高效液相色谱法测定狼毒中狼毒乙素含量的不确定度。方法建立数学模型,对含量测定过程中各影响因素进行分析评估。结果通过计算各变量的不确定度,合成不确定度,狼毒乙素含量的扩展不确定度评估为4.6μg/g。结论 HPLC法测定狼毒药材中狼毒乙素含量的不确定度主要由测定过程中随机效应引入。
OBJECTIVE To evaluate the uncertainty of determination of viridin B in Stellera chamaejasme by high performance liquid chromatography. Methods A mathematical model was established to analyze and evaluate the influencing factors in the determination of content. Results The uncertainty of the synthesis and the uncertainty of the expansion of Copeptin B were estimated to be 4.6 μg / g by calculating the uncertainty of each variable. Conclusion The uncertainty of HPLC method for the determination of Stellera toxins in Stellera chamaejasme is mainly caused by random effects during the measurement.