论文部分内容阅读
目的 :为进一步探讨rGST Sj32保护性免疫机制。 方法 :用rGST Sj32加弗氏完全佐剂 (FCA)皮下免疫BALB/c小鼠。于免疫前 ,攻击感染前 (第 3次免疫后 2wk)以及攻击感染后10d、30d及 4 5d ,各剖杀免疫组与对照组小鼠 5只 ,取脾细胞培养 ,对体外培养的脾细胞诱生的细胞因子进行分析。结果 :用rSj32刺激体外培养的小鼠脾细胞时 ,第 3次免疫后2wk(即攻击感染前 )剖杀小鼠的脾细胞分泌IL 4、IL 5和IFN γ的水平与佐剂对照组相比较 ,均有不同程度的升高 ,分别为 ( 10 .2 1± 3.6 5 )ng/L (P >0 .0 5 )、( 19.89± 9.5 7)ng/L (P >0 .0 5 )、( 5 .0 9± 2 .5 1) μg/L (P <0 .0 1)。攻击感染后10d、30d及 4 5d ,对照组与免疫组IFN γ的水平未见升高 ;对照组IL 4和IL 5的水平随着感染时间的延长明显升高 ,免疫组升高不如对照组明显。结论 :rGST Sj32疫苗免疫BALB/c小鼠后 ,可诱导以Th1类细胞因子为主的免疫应答 ;而攻击性感染则诱导以Th2型细胞因子为主的免疫应答
Objective: To further explore the protective immunity mechanism of rGST Sj32. Methods: BALB / c mice were immunized subcutaneously with rGST Sj32 plus Freund’s complete adjuvant (FCA). Before immunization, before challenge infection (2wk after the third immunization) and 10d, 30d and 45d after the challenge infection, five mice in the immunized group and the control group were killed and the spleen cells were cultured. The cultured spleen cells Induced cytokines were analyzed. RESULTS: When splenocytes cultured in vitro were stimulated with rSj32, the levels of IL 4, IL 5 and IFNγ secreted by splenocytes of mice that were dissected 2wk after the third immunization (ie before challenge infection) were similar to those of the adjuvant control group (10.21 ± 3.6 5) ng / L (P> 0.05), (19.89 ± 9.57) ng / L, P> 0.05, respectively) , (5.90 ± 2.51) μg / L (P <0.01). At 10d, 30d and 45d after challenge, the levels of IFN-γ in the control group and the immunized group did not increase. The levels of IL-4 and IL-5 in the control group increased significantly with the prolongation of infection time, obvious. CONCLUSION: Th1 cytokine-based immune response can be induced by BALB / c mice immunized with rGST Sj32 vaccine, whereas aggressive infection induces a Th2-type cytokine-based immune response