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为了获得超表达獐茅Al HAK1基因棉花纯合系并验证其耐盐能力,对转Al HAK1基因T3代材料进行了PCR与RT-PCR鉴定,并在水培条件下研究了纯合系植株长势、根系形态参数、离子含量和相关抗氧化酶活性变化。结果表明在盐胁迫条件下,转基因植株偏高,较野生型增加了16.5%,同时转基因植株根系形态参数总根长、比根长和根表面积分别增加了62.23%、40.02%和29.22%,而根的平均直径降低了22.22%,均达到了差异显著水平(P<0.05)。此外,在盐胁迫条件下,转基因幼苗能维持根和叶中较高的K+含量,较低的Na+含量和较高的K+/Na+,其超氧化物歧化酶(SOD)和过氧化酶(POD)活性均比野生型植株有所提高。该结果进一步表明供试外源Al HAK1基因在棉花中超表达,提高了转基因棉花耐盐能力,为培育适应盐渍化土壤环境下生长的棉花新品种提供重要的种质资源。
In order to obtain the homozygous lines of Al HAK1 overexpression and verify its salt-tolerance ability, the T3 generation of Al HAK1 gene was identified by PCR and RT-PCR. The growth of homozygous plants , Root morphological parameters, ion content and related antioxidant enzyme activities. The results showed that under salt stress, the transgenic plants were higher than the wild type by 16.5%, while the total root length, root length and root surface area of the transgenic plants increased by 62.23%, 40.02% and 29.22%, respectively The average root diameter decreased by 22.22%, reaching a significant difference (P <0.05). In addition, under salt stress, the transgenic seedlings could maintain higher K + content, lower Na + content and higher K + / Na + content in roots and leaves, and superoxide dismutase (SOD) and peroxidase (POD) ) Activity than the wild-type plants have increased. The results further indicated that the exogenous Al HAK1 gene was overexpressed in cotton and improved the salt tolerance of transgenic cotton, which provided important germplasm resources for breeding new cotton varieties adapted to the growth of salinized soil.