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目的:观察Bmi-1稳定干扰后对乳腺癌细胞药敏性的影响。方法:构建Bmi-1shRNA逆转录病毒表达载体,并用该逆转录病毒感染人乳腺癌细胞,建立Bmi-1稳定干扰的乳腺癌细胞株。采用不同浓度的化疗药物5-氟尿嘧啶和顺铂处理上述细胞,MTT法观察乳腺癌细胞的生长抑制率。结果:免疫印迹检测结果表明Bmi-1稳定干扰的乳腺癌细胞株成功建立。MTT检测显示Bmi-1稳定干扰的乳腺癌细胞(MDA-MB-435S-Bmi-1/RNAi)增殖抑制率显著高于对照细胞(MDA-MB-435S-vector)(P<0.05),并且呈浓度依赖性。其中在MDA-MB-435S-vector中5-氟尿嘧啶和顺铂的IC50值分别为1.7233mg/L和2.9065mg/L,而在MDA-MB-435S Bmi-1/RNAi稳定细胞株5-氟尿嘧啶和顺铂中的IC50值分别为0.9733mg/L和1.8563mg/L(P<0.05)。结论:Bmi-1在乳腺癌细胞对化疗药物敏感性中发挥重要作用,Bmi-1干扰能增加乳腺癌细胞对化疗药物的敏感性。
Objective: To observe the effect of stable Bmi-1 interference on the sensitivity of breast cancer cells. Methods: The Bmi-1 shRNA retrovirus expression vector was constructed. The human breast cancer cell line was infected with the retrovirus to establish a stable Bmi-1-dependent breast cancer cell line. The above-mentioned cells were treated with 5-fluorouracil and cisplatin with different concentrations of chemotherapeutic drugs. The growth inhibition rate of breast cancer cells was observed by MTT assay. Results: Western blotting results showed that Bmi-1 stable interference of breast cancer cell lines was successfully established. The inhibition rate of MDA-MB-435S-Bmi-1 / RNAi cells stably expressing Bmi-1 was significantly higher than that of control cells (MDA-MB-435S-vector) Concentration dependence. The IC50 values of 5-fluorouracil and cisplatin in MDA-MB-435S-vector were 1.7233 mg / L and 2.9065 mg / L, respectively, while the IC50 values of 5-fluorouracil and cisplatin in MDA-MB-435S- The IC50 values for cisplatin were 0.9733 mg / L and 1.8563 mg / L, respectively (P <0.05). Conclusion: Bmi-1 plays an important role in chemosensitivity of breast cancer cells. Bmi-1 interference can increase the sensitivity of breast cancer cells to chemotherapeutics.