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用含TritonX-100及脱氧胆酸钠(TDOC)缓冲液从牛、人脑组织中提取,再用亲和层析技术纯化脑细胞膜抗原,经SDS-PAGE分高蛋白多肽,考马斯亮蓝染色,均显示有18条区带,其分子更为16~124KD之间,证实胎牛脑细胞膜蛋白多肽与人脑组织相似,提示个脑与人脑组织有共同抗原成份,实验表明,用该法提取脑细胞的方法是一种简便可靠的方法,为抗脑抗体的分析研究提供了条件。
The brain cell membrane antigen was purified from the bovine and human brain tissue by using Triton X-100 and sodium deoxycholate (TDOC) buffer. Purified brain cell membrane antigen by SDS-PAGE, high protein peptide by SDS-PAGE, Coomassie blue staining, Showed 18 bands, the molecular more between 16 ~ 124KD, confirmed that fetal bovine brain cell membrane protein and human brain tissue similar, suggesting that brain and human brain tissue common antigen components, experiments show that this method is used to extract The method of brain cells is a simple and reliable method for the analysis of anti-brain antibodies provided the conditions.