Development of EST-SSR primers and their practicability test for Laminaria

• 作 者: WANG Guoliang TAN Xiaolong SHE
• 机 构: CollegeofMarineLifeScience,InstituteofGeneticsandDevelopmentalBiology,CollegeofHorticulture
• 出 处: 海洋学报：英文版
• 发表日期: 2011年3期
• 关键词: EST数据库 SSR引物 测试 海带 DNA模板 开发 遗传多样性 经济海藻 Laminaria EST-SSRs germplasm identifica
• 基金项目: Foundation item： The National Natural Science Foundation of China under contract No. 40776072 and 40606035, the National High Technology Research and Development Program （863 Program） of China under c

Laminaria (Saccharina) is one of the economic important seaweeds.Through sequence analysis of 4 099 ESTs from Laminaria (971 were generated from our own cDNA laboratories and 3 128 were downloaded from updated EST databases) with SSRIT software,two hundre