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近年来,随着微操作研究的不断发展和深入,批量目标微操作成为当前的研究热点之一.由于显微镜视野范围狭小,微操作过程中需要不断进行物镜转换和调焦工作.本文研制的全自动微操作平台将调焦和物镜转换改造为电动方式,实现了自动调焦和物镜自动转换功能.针对细胞图像的特点,本文提出了基于扫描线的归一化方差聚焦测度方法,并在此基础上,设计了相应的聚焦平面搜索策略.针对显微镜物镜转化器的特点,本文设计了自动物镜转换结构和控制方法,实现不同放大倍数物镜的转换.最后本文在细胞图像处理的实验中,构建了具有分层递阶结构的全局视野,验证了自动调焦和物镜自动转换的准确控制,为批量目标操作的自动化奠定了基础.
In recent years, with the continuous development and deepening of micro-operation research, batch target micro-operation has become one of the current research hotspots.As a result of the narrow field of view of the microscope, it is necessary to continuously perform the objective lens conversion and focusing work during the micro-operation. The automatic micro-operation platform converts the focusing and objective conversion into electric mode, and realizes the automatic focusing and automatic conversion of the objective lens.According to the characteristics of cell images, this paper proposes a normalized variance focusing measure method based on scan lines, Based on which a corresponding focal plane search strategy is designed.According to the characteristics of the microscope objective lens converter, this paper designs an automatic objective lens conversion structure and control method to realize the conversion of different magnification objectives.Finally, in the experiment of cell image processing, The global vision with hierarchical and hierarchical structure verifies the accurate control of autofocusing and automatic conversion of objective lens, which lays the foundation for the automation of batch target operation.