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观察了三种不同质的动、植物蛋白质组成的含蛋白质17%的饲料对运动大鼠蛋白质及能量代谢的影响。实验取雄性断轧幼鼠32只,随机等分为A、B、C、D四组。饲料中蛋白质组成:A组为100%麦蛋白;B组酪蛋白10%,麦蛋白90%;C及D组均为酪蛋白40%,麦蛋白60%。A、B、C组为观察不同质蛋白质对运动大鼠蛋白质及能量代谢的影响,D组为C组的安静对照组。观察指杯有体重、氮平衡、骨骼肌氮、血清尿素氮、血糖、血乳酸、肌糖元、肌轧酸脱氢酶、肌ATP和肌Ca2+-ATP酶活性。结果表明:1.不同质蛋白影响大鼠蛋白质代谢。A组蛋白质最差、运动后大鼠呈负氮平衡,且血清尿素氮含量较高,肌氮较低,表明蛋白质分解代谢增强。2.不同质蛋白也影响大鼠能量代谢,C组蛋白质较好,运动时消耗了较多的肌糖原,产生较多的ATP;LDH和Ca2-ATP酶活性较高。3.相同质蛋白的C、D组,运动的C组在能量的产生及酶活性等方面,其数值稍高于安静对照的D组。4.从蛋白质及能量代谢看,以酪蛋白与麦蛋白4:6为适宜。
The effects of three different kinds of animal protein and animal protein containing 17% feed on the protein and energy metabolism of rats were observed. Twenty-two male offspring offspring were randomly divided into four groups: A, B, C and D. Protein composition of feed: A group of 100% wheat protein; B casein 10%, 90% of wheat protein; C and D were 40% casein, wheat protein 60%. Groups A, B, and C were observed for the effect of different proteins on protein and energy metabolism in exercise-induced rats. Group D was a quiet control in group C. Observe the finger cup weight, nitrogen balance, skeletal muscle nitrogen, serum urea nitrogen, blood glucose, blood lactate, muscle glycogen, anacardic acid dehydrogenase, muscle ATP and muscle Ca2 + -ATPase activity. The result shows: 1. Different Proteins Affect Protein Metabolism in Rats. The protein in group A was the worst. After exercise, the rats showed a negative nitrogen balance, and the serum urea nitrogen content was higher and the muscle nitrogen was lower, indicating that protein catabolism was enhanced. 2. Different proteomics also affect the energy metabolism in rats. The protein in group C is better, more muscle glycogen is consumed during exercise, more ATP is produced, and LDH and Ca2-ATPase activities are higher. 3. The same quality protein C, D group, exercise C group in terms of energy production and enzyme activity, the value slightly higher than the quiet control group D. 4. From protein and energy metabolism, casein and wheat protein 4: 6 is suitable.