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用逆转录-聚合酶链反应法(PT-PCR)检测了67例原发性肝癌(PLC)患者血清中的庚型肝炎病毒(HGV)RNA,以PCR-双脱氧末端终止法分析了PCR产物的核苷酸序列。结果显示,HGVRNA的检出率为19.4%(13/67),其在HBsAg阳性组和HBsAg/抗HCV阴性组中的阳性率分别是12.2%(6/49)和50%(7/14);5’非编码区(5’-NCR)扩增片段的核苷酸序列与美国株GBV-C的同源性为91.7%,提示在我国PLC患者中存在HGV感染。
Mitochondrial hepatitis virus (HGV) RNA in serum of 67 patients with primary liver cancer (PLC) was detected by reverse transcription-polymerase chain reaction (PT-PCR) and PCR products were analyzed by PCR-dideoxy end termination method. The nucleotide sequence. The results showed that the detection rate of HGVRNA was 19.4% (13/67), and the positive rates in the HBsAg positive group and the HBsAg/anti-HCV negative group were 12.2% (6/49) and 50%, respectively ( 7/14); The nucleotide sequence of the 5’-NCR amplified fragment was 91.7% homologous to the US strain GBV-C, suggesting that HGV infection exists in PLC patients in China.