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目的:研究穿琥宁在人体内的药代动力学过程并建立人生物样品的高效液相色谱方法。方法:采用液-液萃取的方法从生物样品中提取出穿琥宁后用Diaminsil-C18(2)色谱柱(250 mm×4.6 mm,5μm)柱进行分离,采用的流动相为:乙腈-磷酸盐缓冲液[0.5 g.L-1磷酸二氢钾,用磷酸调节pH至2.5(±0.05)](43∶57,v/v)。结果:穿琥宁在人体内静脉给药200 mg后药代动力学符合二室模型,统计距参数为:Cmax为(9.796±2.306)μg.mL-1、Tmax(1.382±0.231)h、T1/2z(1.678±0.509)h。结论:此方法快捷、灵敏、简便,适合于穿琥宁的药代动力学研究。
OBJECTIVE: To study the pharmacokinetics of Chuanhuning in human and to establish a HPLC method for human biological samples. METHODS: Chitosan was extracted from biological samples by liquid-liquid extraction and then separated on a Diaminsil-C18 (2) column (250 mm × 4.6 mm, 5 μm). The mobile phases were acetonitrile-phosphate buffer [0.5 gL-1 potassium phosphate monobasic, adjusted to pH 2.5 (± 0.05) with phosphoric acid] (43:57, v / v). Results: The pharmacokinetics of chuanhuning after intravenous administration of 200 mg in human body accorded with two-compartment model. The statistical distance parameters were as follows: Cmax was (9.796 ± 2.306) μg.mL-1, Tmax was 1.382 ± 0.231 h, T1 / 2z (1.678 ± 0.509) h. Conclusion: This method is fast, sensitive and simple, suitable for chuanhuning pharmacokinetic study.