Control of gallbladder contractions by cholecystokinin through cholecystokinin-A receptors on gallbl

• 作 者: Dan Xu Bao-Ping Yu He-Sheng Luo Ling-Dan Chen
• 机 构: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, C
• 出 处: 世界胃肠病学杂志（英文版）
• 发表日期: 2008年18期
• 关键词: Gallbladder Interstitial cells of cajal Cholecystokinin-A receptor C-kit Immunoh

AIM: To identify the cholecystokinin (CCK)-A receptors (CCK-AR) on the guniea pig gallbladder interstitial cells of cajal (ICC) and to study CCK-8 induced gallbladder muscle strip contractions through the CCK-AR.METHODS: The existence of CCK-AR was examined by immunohistofluorescence on sectioned tissue and cultured cells. In vitro contractile response of guinea pig gallbladder muscle strips and the strips with ICC removed were also studied with CCK-8 receptors added.RESULTS: In tissue sections, intensely CCKARimmunoreactive interstitial cells were found mainly in the muscular layers. In cultured cell sections, distinctive double staining of C-kit and CCK-AR ICCs were found.When we removed the ICC of the gallbladder, CCK-8induced muscle strip contraction dose response curve significantly shifted to the right.CONCLUSION: We proved that both the existence of CCK-AR on the guinea pig gallbladder ICC and CCK evoked contraction are mediated through direct action on CCK-AR on the gallbladder ICC.