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对粘虫胚胎细胞的继代培养条件进行了研究。结果表明,TC-100(SIGMA)培养基最适合细胞生长。不同血清浓度和pH的培养基对细胞生长影响很大,以10%小牛血清浓度和pH6.2的培养基对细胞传代培养最为适宜。细胞生长依赖于细胞接种密度,以 5×10~5细胞/mL细胞接种密度对细胞生长最为适宜。适于细胞培养的最适温度范围是26-29℃。
The subculture conditions of armyworm embryonic cells were studied. The results show that TC-100 (SIGMA) medium best suited for cell growth. Different serum concentrations and pH of the medium on the cell growth of a great influence to 10% fetal bovine serum concentration and pH 6.2 medium most suitable for cell subculture. Cell growth depends on the cell seeding density, and cell density of 5 × 10 5 cells / mL is most suitable for cell growth. The optimum temperature range for cell culture is 26-29 ° C.