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目的:研究脾气虚证大鼠血管紧张素Ⅱ(AngⅡ)、内皮素1、一氧化氮(NO)的变化及健脾益气中药对其的影响。方法:实验动物分成空白组、模型组、四君子汤组、六君子汤组、归脾汤组。分别采用ELISA法、硝酸还原酶法检测各组大鼠血浆中AngⅡ、ET-1,血清中的NO含量。结果:与空白组比较,其他各组的AngⅡ、ET-1含量升高,NO含量降低;与模型组比较,中药治疗组的AngⅡ、ET-1含量降低明显,NO含量升高明显。结论:脾气虚证大鼠血浆中AngⅡ、ET-1含量升高,NO含量降低,而健脾益气中药能改善这一情况,为健脾益气中药可防治心脑血管疾病提供实验室依据。
Objective: To investigate the changes of angiotensin Ⅱ (Ang Ⅱ), endothelin-1 and nitric oxide (NO) in rats with spleen-qi deficiency syndrome and the effect of Jianpi Yiqi Herbs on them. Methods: The experimental animals were divided into blank group, model group, Sijunzi Decoction group, Liujunzi Decoction group and Guipi Decoction group. The levels of AngⅡ, ET-1 and NO in serum were detected by ELISA and nitrate reductase method respectively. Results: Compared with the blank group, the contents of AngⅡ and ET-1 in the other groups were increased and the content of NO was decreased. Compared with the model group, the content of AngⅡ and ET-1 in the TCM group decreased significantly and the content of NO increased significantly. Conclusion: The content of AngⅡ and ET-1 in the plasma of spleen-qi deficiency rats is increased and the content of NO is decreased, while the TCM of Jianpi Yiqi can ameliorate the situation and provide the laboratory evidence for the prevention and treatment of cardiovascular and cerebrovascular diseases .