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目前,晶状体本身的代谢异常与白内障发病的关系愈来愈引起人们的重视,许多学者认为晶状体某些酶活性下降及光化学反应引起晶状体的超氧化损伤是老年性白内障发病的重要因素。6(?)磷酸葡萄糖脱氢酶(G6PD)、乳酸脱氢酶(LDH)及苹果酸脱氢酶(MDH)从三个不同的代谢途径(磷酸戊糖途径、无氧酵解及三羧循环途径)反映了整个晶体糖代谢状态,并且晶状体上皮细胞是晶状体代谢最活跃的部位,是整个晶状体代谢的关键,它的形态与功能的正常与否是维持晶状体透明性的重要条件,目前尚未见到采用细胞化学方法定量测定晶状体酶活性的报道。为此,我们用这种方法显示酶的活性,然后用MPV-TASPLUS 显微分光图象分析仪对各种不同类型的白内障晶状体上皮
At present, the relationship between the metabolism of lens itself and the incidence of cataract more and more attracted people’s attention, many scholars believe that some of the lens activity decreased and photochemical reactions caused by lens superoxide damage is an important factor in the incidence of senile cataract. (?) Glucose phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) from three different metabolic pathways (pentose phosphate pathway, anaerobic glycolysis and tricarboxylic acid cycle Pathway) reflects the entire crystalline glucose metabolism, and lens epithelial cells are the most active part of the lens metabolism, the key to the entire lens metabolism, its morphology and function of the normal or not is to maintain the transparency of the lens important conditions, has not yet seen To the use of cytochemical methods to quantify the activity of the lens enzyme reported. For this purpose, we used this method to show the activity of the enzyme and then analyzed the different types of cataract lens epithelium using the MPV-TASPLUS spectrophotometer