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目的:研究胰岛素样生长因子-1(IGF-1)在2型糖尿病合并结肠癌患者血清及癌组织中的表达,探讨其在2型糖尿病并发结肠癌中的作用。方法:采用放射免疫测定法及免疫组化SP染色法检测40例2型糖尿病合并结肠癌患者(A组)、30例2型糖尿病合并结肠腺瘤患者(B组)、20例结肠癌病患者(C组)、20例2型糖尿病患者(D组)及健康对照者(E组)血清IGF-1水平及其在组织中的表达。结果:5组间血清IGF-1水平差异有统计学意义(F=14.577,P<0.01)。组间两两比较结果提示A组血清IGF-1水平高于其他4组(P<0.05),E组血清IGF-1水平低于其它4组(P<0.05)。与正常结肠黏膜组比较,结肠腺瘤组、结肠癌组IGF-1的表达增强,差异有统计学意义(P<0.01);与结肠腺瘤组比较,IGF-1在结肠癌中的表达显著增强(P<0.05)。结论:2型糖尿病并发结肠癌的过程中,IGF-1可能发挥了一定作用。
Objective: To investigate the expression of insulin-like growth factor-1 (IGF-1) in serum and cancer tissues of type 2 diabetes mellitus patients with colon cancer and to explore its role in type 2 diabetes complicated with colon cancer. Methods: Forty patients with type 2 diabetes mellitus complicated with colon cancer (group A), 30 type 2 diabetes mellitus complicated with colorectal adenoma (group B) and 20 patients with colon cancer were examined by radioimmunoassay and immunohistochemical SP staining. (Group C), 20 cases of type 2 diabetes (group D) and healthy control (group E) serum IGF-1 levels and its expression in the tissue. Results: There was a significant difference in serum IGF-1 levels between the five groups (F = 14.577, P <0.01). The result of pairwise comparisons between two groups showed that serum IGF-1 level in group A was higher than that in other four groups (P <0.05), serum IGF-1 level in group E was lower than the other four groups (P <0.05). Compared with normal colonic mucosa group, the expression of IGF-1 in colon adenocarcinoma group and colon cancer group was significantly increased (P <0.01). Compared with colon adenoma group, the expression of IGF-1 in colorectal carcinoma was significantly Enhanced (P <0.05). Conclusion: IGF-1 may play a role in the development of type 2 diabetes mellitus complicated with colon cancer.