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本文研究建立了人血清中氯林可霉素的高效液相色谱测定法（ＨＰＬＣ），该方法在超低紫外（１９８ｎｍ）下测定，具有取样量小（２００μｇ），线性范围宽（０．５％～５０μｇ／ｍｌ）；灵敏度高，以３倍基线噪音计算，最低检测限可达０．０５μｇ／ｍｌ；重现性好，高中低３个质控样品批间ＲＳＤ％分别为１．９７、０．４０和２．１５，批内ＲＳＤ％分别为０．８０、０．４６和２．３３，平均回收率分别为１０１．８７，１００．３４％和９３．８１％；方法操作简便等特点，为测定血清中药物浓度，确定给药方案和治疗剂量，以及药物动力学分析和药效学评价提供了方法学基础
In this paper, a high performance liquid chromatography (HPLC) method was developed for the determination of clindamycin in human serum. The method was developed for the determination of clindamycin in human serum with a small sample volume (200μg) and a linear range of 0.5 % ~ 50μg / ml). The sensitivity was high and the detection limit was up to 0.05μg / ml with 3 times baseline noise. The RSD% of the three quality control samples with good reproducibility and high school low were 1.97, 0.40 and 2.15 respectively. The intra-assay RSD% were 0.80, 0.46 and 2.33, respectively. The average recoveries were 101.87, 100.34% and 93.81% respectively. The method was simple and easy to operate , Providing a methodological basis for the determination of drug concentration in serum, determination of dosing regimens and therapeutic doses, and pharmacokinetic analysis and pharmacodynamic evaluation