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目的 探讨粘附在Cytodex-1微载体上的MC3T3-E1细胞是否可通过大梯度强磁场实现抗磁悬浮细胞培养,以及在该培养方式下不同模拟重力环境对细胞的生物学影响.方法 鼠颅骨成骨细胞系MC3T3-E1接种于Cytodex-1微载体,将细胞置于大梯度强磁场的表观重力为μG,1G和2G位置,对应磁场强度分别为12T,16 T和12 T,检测细胞形态、增殖、凋亡和周期.结果 μG和1G环境促进了MC3T3-E1细胞增殖,而2G重力环境抑制了细胞增殖;大梯度强磁场环境诱导了细胞形态的改变、增加了细胞的caspase-3活性,影响了细胞周期.结论 粘附在Cytodex-1微载体上的MC3T3-E1细胞在大梯度强磁场环境下可以实现抗磁悬浮培养,大梯度强磁场对细胞的形态和功能均具有较明显的影响.“,”Objective To detect if MC3T3-E1 osteoblast adhered to Cytodex-1 micro-carrier could realize diamagnetic levitation cultivation in large gradient high magnetic field (LGHMF) to simulated weightlessness environment and to determine if MC3T3-E1 osteoblast cell biological responses were affected with this cultivation form under various simulated gravity environments by LGHMF.Methods The murine calvarial osteoblast cell line MC3T3-E1 adhered to Cytodex-1 micro-carrier was subjected to an apparent gravity of μG(12 T),1 G (16 T) and 2 G(12 T) in a LGHMF,respectively.The cell morphology,proliferation,apoptosis and cycle were detected.Results The proliferation of MC3T3-E1 was inhibited in 2 G condition and was promoted in μG and 1 G condition.The LGHMF induced the morphologic change of MC3T3-E1,increased caspase-3 activity and affected the cell cycle.Conclusion MC3T3-E1 adhered to Cytodex-1 micro-carrier could realize diamagnetic levitation and the cells morphology and functions were affected by LGHMF.