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目的探讨心脑舒通胶囊对脑缺血再灌注大鼠脑微循环障碍干预作用。方法采用线栓法制备大鼠局灶性脑缺血再灌注模型,24 h后处死取材,HE染色光镜下观察大鼠皮层区病理形态学变化和损伤状况;放射免疫法检测患侧皮层6-酮-前列腺素F1α(6-Keto-PGF1α)、血栓素B2(TXB2)水平;凝血酶法检测血浆纤维蛋白原(FIB)水平。结果缺血再灌注模型组患侧皮层有明显缺血性病理形态学改变,如神经细胞肿胀、血管内皮细胞肿胀、微血管管腔狭窄等;皮层6-Keto-PGF1α、TXB2水平异常升高(P<0.05,P<0.01);血浆FIB水平显著上升(P<0.01)。心脑舒通胶囊能明显减轻模型大鼠皮层病理形态学损伤;并可降低TXB2水平,降低血浆FIB水平(P<0.01,P<0.05)。结论心脑舒通胶囊可明显减轻缺血再灌注脑组织损伤,保护受损脑组织,其机理可能与调节血管活性物质、调整血液流变学病理状态、从而改善微循环障碍有关。
Objective To investigate the effects of Xinnaoshutong Capsule on cerebral microcirculatory disturbance in rats with cerebral ischemia-reperfusion. METHODS: Focal cerebral ischemia-reperfusion models were established by suture method. The rats were sacrificed 24 hours later. HE staining was used to observe the pathological changes and damage of the cortex in rats. Radioimmunoassay was used to detect the ipsilateral cortex. Keto-prostaglandin F1α (6-Keto-PGF1α) and thromboxane B2 (TXB2) levels; plasma fibrinogen (FIB) levels were measured by thrombin method. RESULTS: Ischemic reperfusion model group had obvious ischemic pathological changes in the affected side cortex, such as swelling of nerve cells, swelling of vascular endothelial cells, and stenosis of microvascular lumens; abnormal levels of 6-Keto-PGF1α and TXB2 in cortex (P). <0.05, P<0.01); plasma FIB levels increased significantly (P<0.01). Xinnaoshutong capsule can significantly reduce the cortical pathological damage of model rats; it can also reduce TXB2 level and plasma FIB level (P<0.01, P<0.05). Conclusion Xinnaoshutong capsule can significantly reduce cerebral ischemia-reperfusion injury and protect damaged brain tissue. The mechanism may be related to the regulation of vasoactive substances, adjustment of hemorheological pathological conditions, and improvement of microcirculation.