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目的用薄层层析和紫外分析方法对纯蛇粉胶囊中所含的乌梢蛇成分进行分析鉴别。方法以中药乌梢蛇作为对照品,以硅胶G铺板,在丙酮、乙醇、氨水和正丁醇、乙醇、氨水2个系统中分别展开,后置于紫外254nm下检视,同法作空白试验。结果在同一薄层色谱中,和对照品相同的位置上,显清晰分离很好的4个斑点,其中2个斑点呈强烈的荧光。空白阴性对照品无此斑点。结论该方法具有定性检查的鉴别意义,为制定纯蛇粉胶囊质量标准提供了实验方法和依据。
Objective To analyze and identify the components of Radix snake snake contained in pure snake capsule by thin layer chromatography and UV analysis. Methods The Chinese black snake snake as a reference substance, with silica gel G decking, in acetone, ethanol, ammonia and n-butanol, ethanol, ammonia two systems were opened, then placed under UV 254nm view, the same method as a blank test. Results In the same TLC, at the same position as the control, four spots clearly separated and two spots were strongly fluorescent. Blank negative control without this spot. Conclusion The method has the significance of qualitative identification, providing experimental methods and basis for the development of pure snake powder capsule quality standards.