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骨髓多染红细胞微核试验(MT)具有简易、快速和敏感的特点,但存在着不能结合亚慢性或慢性毒性试验观察微核细胞率动态变化的缺点。本文试图以外周血微核正染红细胞千分率(MNNCE率)及微核多染红细胞千分率(MNPCE率)为指标,检测已知断裂剂环磷酰胺(CP)的断裂作用,弥补MT的不足,报道如下。取健康雌雄小鼠各20只,按性别随机分入CP5、10、20和40mg/kg四个剂量组和一个生理盐水对照组。动物隔天腹腔注射CP或生理盐水一次共八次。定
Bone marrow erythrocyte micronucleus test (MT) has the characteristics of simple, rapid and sensitive, but there is a shortcoming of not being able to observe the dynamic change of micronuclei rate in sub-chronic or chronic toxicity test. This article attempts to peripheral blood micronuclei micronuclei MNNCE rate (MNNCE rate) and MN micronuclei erythrocyte fraction (MNPCE rate) as an indicator to detect the breaking agent known cyclophosphamide (CP) of the fracture, make up the MT Insufficient, reported as follows. Twenty healthy male and female mice were randomly divided into four groups (CP5, 10, 20 and 40 mg / kg) and a saline control group according to sex. The animals were injected intraperitoneally with CP or normal saline once a day for a total of eight times. set