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目的观察酸性鞘磷脂酶(ASMase)在镉性肾损害过程中的活性变化及规律,探讨ASMase活性变化与镉性肾损害的关系。方法 60只SD雄性大鼠随机分为对照组和实验组,实验组动物腹腔注射1mg/kg Cd2+,每天1次、每周5次,对照组注射等体积的生理盐水。染毒第0、1、3、5和7周末实验组、对照组每组各随机处死6只动物,收集血、尿及肾组织。ELISA法测尿β2微球蛋白的含量,高效液相色谱法测血、尿及肾组织中ASMase活性,制作病理切片观察肾组织病变情况。结果随染毒时间的延长,肾组织有不同程度的病理改变。与对照组相比,实验组动物肾脏系数随染毒时间的延长而增加(P<0.05);尿β2微球蛋白含量随染毒时间的延长而增加(P<0.05);血、尿、肾组织匀浆上清液中ASMase活性从染毒第3周开始随染毒时间的延长而增加(P<0.05)。结论镉性肾损害中ASMase活性随染毒时间的延长而增加。
Objective To observe the changes and regularity of activity of acidic sphingomyelinase (ASMase) in the process of cadmium-induced renal damage and to explore the relationship between the changes of ASMase activity and cadmium-induced renal damage. Methods Sixty male Sprague Dawley rats were randomly divided into control group and experimental group. Animals in the experimental group were intraperitoneally injected with 1 mg / kg Cd2 + once a day for 5 times a week. The control group was injected with equal volume of normal saline. On the 0th, 1st, 3rd, 5th and 7th week of exposure, six animals in each experimental group and control group were randomly sacrificed to collect blood, urine and kidney tissue. The content of urinary β2-microglobulin in urine was measured by ELISA, the activity of ASMase in blood, urine and renal tissues were detected by high performance liquid chromatography (HPLC). Pathological changes of renal tissue were observed. Results With the extension of exposure time, the renal tissue had different degrees of pathological changes. Compared with control group, the kidney coefficient of experimental group increased with the prolongation of exposure time (P <0.05); the content of urinary β2 microglobulin increased with the prolongation of exposure time (P <0.05); the blood, urine and kidney The ASMase activity in the tissue homogenate supernatant increased with the prolongation of exposure time from the third week of exposure (P <0.05). Conclusion The activity of ASMase in cadmium-induced renal damage increased with the prolongation of exposure time.