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目的观察八宝丹对二甲基亚硝胺(DMN)诱导的大鼠肝纤维化的干预作用。方法 90只SD雄性大鼠随机分为对照组和造模组,以0.5%DMN按2μl/g体质量腹腔注射4周复制大鼠肝纤维化模型。4周末造模组大鼠随机分为模型组,八宝丹高、中、低剂量组,扶正化瘀组;用药各组灌胃相应药物,正常组和模型组灌胃等剂量的0.5%羧甲基纤维素钠(CMC),给药4周。8周末处死动物,留取标本。检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性和白蛋白(ALB)、总胆红素(TBIL)含量;测定肝组织中羟脯氨酸(Hyp)含量;HE染色和天狼猩红染色观察肝组织病理学改变。结果与正常组比较,模型组血清TBIL、ALT、AST水平明显升高(P<0.05),各药物组干预后,血清学指标TBIL、ALT、AST均有所降低;HE染色显示模型组肝脏内有大量炎性细胞浸润、肿胀、变性坏死,有明显出血现象;天狼猩红染色显示模型组大鼠肝脏胶原增生明显,形成假小叶,胶原面积明显增加;模型组肝组织Hyp含量较正常组明显升高(P<0.01),药物干预后肝组织炎症和纤维化程度较模型组均有所改善,肝组织羟脯氨酸含量明显降低,高、中剂量八宝丹组和扶正化瘀组较模型组肝组织胶原沉积明显减轻(P<0.05),扶正化瘀组与八宝丹高剂量组比较差异无统计学意义(P>0.05)。结论八宝丹对DMN诱导的大鼠肝纤维化有较好的逆转作用,高剂量八宝丹的疗效相对于中低剂量的八宝丹更为突出;八宝丹高剂量组与阳性对照药物扶正化瘀胶囊组对大鼠肝纤维化的干预效果类似。
Objective To observe the effect of Babaidan on rat hepatic fibrosis induced by DMN. Methods Ninety SD male rats were randomly divided into control group and model group. Rat models of hepatic fibrosis were induced by intraperitoneal injection of 0.5% DMN at 2μl / g body weight for 4 weeks. Rats in model group were randomly divided into model group, Babao Dan high, medium and low dose groups and Fuzhenghuayu group. The rats in each group were given gavage with the corresponding drugs, normal group and model group by intragastric administration of 0.5% Sodium methylcellulose (CMC) for 4 weeks. Animals were sacrificed 8 weeks later, specimens were taken. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and albumin (ALB) and total bilirubin (TBIL) were measured. Hyp contents in liver tissue were measured. HE staining and Sirius red Staining observed liver histopathological changes. Results Compared with the normal group, the levels of serum TBIL, ALT and AST in the model group were significantly increased (P <0.05), and the serum TBIL, ALT and AST decreased after intervention in each drug group. HE staining showed that the intrahepatic A large number of inflammatory cell infiltration, swelling, degeneration and necrosis, significant hemorrhage; Sirius red staining showed that the model group rats liver collagen hyperplasia, the formation of false lobules, collagen area increased significantly; model group liver Hyp levels were significantly higher than the normal group (P <0.01). The degree of inflammation and fibrosis of liver tissue in the model group were improved after drug intervention, and the content of hydroxyproline in liver tissue was significantly decreased Collagen deposition in the model group was significantly reduced (P <0.05), while there was no significant difference between the Fuzhenghuayu group and the Babao Dan high-dose group (P> 0.05). Conclusion Babaidan DMN-induced rat liver fibrosis has a good reversal of the effect of high-dose eight dipyridamole relative to low-dose eight-dipyridamole more prominent; Babao Dan high-dose group and positive control drug Fuzhenghuayu Capsule intervention in rats with similar effects of liver fibrosis.