论文部分内容阅读
目的:探讨应用RNA i技术下调survivin基因对人卵巢癌SKOV3细胞增殖、凋亡及顺铂敏感性的影响。方法:构建survivin基因shRNA真核表达载体,转染人卵巢癌SKOV3细胞。定量PCR和Western blotting观察SKOV3细胞survivin mRNA和蛋白表达的改变;噻唑蓝(MTT)检测细胞增殖活性和药物敏感性;流式细胞仪检测细胞凋亡。结果:SKOV3-siRNA组细胞survivin mRNA及蛋白表达下降,同时细胞增殖能力明显降低,细胞凋亡率显著增加(P<0.05)。SKOV3-siRNA组细胞对顺铂的化学敏感性升高,顺铂的IC50值降低(P<0.05)。结论:下调survivin基因表达能够抑制卵巢癌SKOV3细胞增殖能力,诱导细胞凋亡,增强细胞顺铂药物敏感性。因此,survivin基因可能成为抗肿瘤治疗的潜在靶点。
Objective: To investigate the effect of down-regulation of survivin gene on the proliferation, apoptosis and cisplatin sensitivity of human ovarian cancer SKOV3 cells by RNAi technique. Methods: The survivin gene shRNA eukaryotic expression vector was constructed and transfected into human ovarian cancer SKOV3 cells. Quantitative PCR and Western blotting were used to detect the expression of survivin mRNA and protein in SKOV3 cells. Cell proliferation and drug sensitivity were detected by MTT assay. Cell apoptosis was detected by flow cytometry. Results: The expression of survivin mRNA and protein in SKOV3-siRNA group decreased significantly, while the proliferation of SKOV3-siRNA group was significantly decreased (P <0.05). The chemosensitivity of cisplatin to SKOV3-siRNA cells was increased, and the IC50 of cisplatin was decreased (P <0.05). Conclusion: The down-regulation of survivin gene expression can inhibit the proliferation of ovarian cancer SKOV3 cells, induce apoptosis and enhance the sensitivity of cisplatin to cisplatin. Therefore, the survivin gene may become a potential target for anti-tumor therapy.