论文部分内容阅读
目的 :研究SLE患者外周血单个核细胞 (PBMCs)中淋巴细胞亚群的凋亡特点。方法 :采用三色荧光流式细胞术检测CD3、CD4、CD8、CD19细胞亚群的早期凋亡 ;ELISA法检测PBMCs培养上清中的sFas、sFasL ;实时荧光半定量RT PCR方法检测细胞内FasmRNA的表达水平。结果 :SLE患者PBMCs中CD3+ 细胞凋亡明显增多 ;CD4 + 和CD8+ 细胞亚群凋亡均有明显增加 ,但以CD4 + 细胞凋亡增多更为明显 ;相应地 ,其PBMCs中CD4 + 、CD8+ 细胞百分率下降 ,CD4 CD8比值降低。SLE患者血清可诱导CD3+ 、CD4 + 、CD8+ T细胞亚群凋亡增多 ,sFas、sFasL水平增高及FasmRNA表达增加 ;抗 IL 10抗体则可中和SLE血清的上述作用。结论 :SLE患者体内T细胞凋亡增多 ,以CD4 + T细胞凋亡增加更为显著 ,导致CD4 CD8比值下降 ;SLE血清中高水平的IL 10可能通过诱导Fas、FasL表达增高而促进T细胞凋亡的发生。
Objective: To study the apoptosis of lymphocyte subsets in peripheral blood mononuclear cells (PBMCs) from patients with SLE. Methods: The early apoptosis of CD3, CD4, CD8 and CD19 cell subsets was detected by three-color fluorescence flow cytometry. The sFas and sFasL were detected by ELISA in the culture supernatant of PBMCs. The expression of Fas mRNA The level of expression. Results: The apoptosis of CD3 + cells in PBMCs was significantly increased in SLE patients. The apoptosis of CD4 + and CD8 + cells was significantly increased in SLE patients, but the apoptosis of CD4 + cells was more obvious. Correspondingly, CD4 +, CD8 + The percentage decline, CD4 CD8 ratio decreased. SLE serum induced CD3 +, CD4 +, CD8 + T cell subsets increased apoptosis, sFas, sFasL levels increased and Fas mRNA expression increased; anti-IL10 antibody can neutralize the role of SLE serum. CONCLUSIONS: In SLE patients, the apoptosis of T lymphocytes is increased and the apoptosis of CD4 + T cells is more obvious, leading to the decrease of CD4 CD8 ratio. The high level of IL 10 in SLE serum may promote the apoptosis of T cells by inducing the increase of Fas and FasL happened.