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本文报道以抗盘尾丝虫Onchocerca gibsoni的单克隆抗体Gib 13株,双面免疫放射计量试验(IRMA)检测斯里兰卡和巴布亚-新几内亚的班氏丝虫病人血清循环抗原。取BALB/c小鼠,按10~4/鼠接种O.gibsoni胚卵及等量的微丝蚴,16天后再用同样数量的上述抗原静注强化免疫,然后以BALB/c小鼠脾细胞与NS-1骨髓瘤细胞融合。间接免疫荧光抗体法(IFAT)筛选分泌抗牛丝虫微丝蚴表面抗原的杂交瘤株。经两次克隆后,注入BALB/c小鼠,收集腹水。5%硫酸铵沉淀腹水中的单克隆抗体并透析,IFAT测定所获的各单克隆抗体对班氏丝虫微丝蚴表
This article reports the detection of serum circulating antigens in Bancroftian filariasis patients in Sri Lanka and Papua New Guinea using the monoclonal antibody Gib 13 against Inchocerca gibsoni and the double-sided immunoradiometric assay (IRMA). BALB / c mice were inoculated with O.gibsoni embryos eggs at 10-4 / mouse and microfilariae of the same size. After 16 days, mice were immunized intravenously with the same amount of the above antigens and then immunized with BALB / c mouse spleen cells Fusion with NS-1 myeloma cells. Indirect immunofluorescence antibody method (IFAT) was used to screen hybridoma strains that secreted antigens of anti-filarial microfilariae surface antigens. After two clones, BALB / c mice were injected and ascites was collected. 5% ammonium sulfate precipitated monoclonal antibody in ascites and dialysis, IFAT determination of each monoclonal antibody to filariasis of Bancrofoo