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采用正交设计考察了液体摇瓶培养平菇菌种过程中,培养基C/N、pH、摇床振荡频率、光照强度等理化因子对菌丝生长量的影响,建立有参考价值的液体摇瓶培养平菇菌种的培养条件指标:摇床振荡频率90r/min(往复式摇床),培养基碳氮比50～70:1,pH5～7,黑暗条件。对液体摇瓶过程中糖的作用及培养液pH的变化进行了研究。通过液体菌种和固体菌种应用的对比试验,建议菌种厂推广液体摇瓶培养技术生产菌种,并将液体菌种和固体菌种结合应用,发挥液体菌种菌龄一致、生活力强和固体菌种保存期长的优点。
The effects of physical and chemical factors such as medium C / N, pH, shaker oscillation frequency and light intensity on the mycelium growth were investigated by orthogonal design. The establishment of a reference value of the liquid shake Flask cultivation of mushroom strains culture conditions indicators: shaker oscillation frequency 90r / min (reciprocating shaker), the medium carbon and nitrogen ratio 50 ~ 70: 1, pH5 ~ 7, dark conditions. The effect of sugar in liquid shake flask and the change of pH of culture medium were studied. Through the comparison test between the application of liquid and solid strains, it is suggested that the strain plant popularize liquid shake flask culture technology to produce the strain, and combine the liquid strain and the solid strain to exert the same bacteria age and strong viability And the advantages of long storage period of solid bacteria.