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目的研究间隔区寡核苷酸序列(Spoligotyping)基因分型技术在河南省结核病分枝杆菌基因分型中的应用,了解河南省基因型种类和分布以及北京家族基因型在河南省的分布特征。方法应用2007年在河南省结核病防治机构实验室分离培养的310株结核分枝杆菌,设计引物,应用PCR和间隔区寡核苷酸分型(Spoligotyping)技术对结核分枝杆菌进行分型检测分析,基因分型分析经http://www.miru-vntrplus.org/MIRU/index.faces网站分析,统计学数据经卡方检验。结果对310株结核分枝杆菌临床分离株的Spoligotyping结果显示分为4个基因群(Beijing和Beijing近似群、T群、Manu群、S群),24个基因型,有267株为北京家族基因型,占86.1%,且北京家族基因型与MDR耐药有相关性;河南省较年轻人群(<60岁)感染的结核分枝杆菌中,北京家族基因型所占比例高于年老组(即>60岁组),北京家族基因型与年轻组的联系强于年老组;北京家族基因型的分布在不同的地区间有差异。结论河南省结核分枝杆菌以北京家族基因型为主要流行型,且与耐药及年龄、地域有相关性,重点监测北京家族基因型。
Objective To study the genotyping of Mycobacterium tuberculosis in Henan Province using genotyping of Spoligotyping and find out the genotype and distribution of genotypes in Henan Province and the distribution characteristics of Beijing genotypes in Henan Province. Methods 310 strains of Mycobacterium tuberculosis were isolated and cultured in the Laboratory of Tuberculosis Prevention and Control of Henan Province in 2007. The primers were designed and the genotypes of Mycobacterium tuberculosis were detected by PCR and Spoligotyping The genotyping analysis was analyzed by http://www.miru-vntrplus.org/MIRU/index.faces, and the statistical data were analyzed by chi-square test. Results The Spoligotyping results of 310 clinical isolates of Mycobacterium tuberculosis showed that there were 4 gene clusters (Beijing and Beijing approximation population, T population, Manu population and S population), 24 genotypes and 267 strains were Beijing family genes Type, accounting for 86.1%, and Beijing family genotypes were associated with MDR resistance. Among the younger population (<60 years old) infected with Mycobacterium tuberculosis in Beijing, the proportion of Beijing family genotypes was higher than that of the elderly group That is,> 60-year-old group), Beijing family genotypes were stronger than those in the young group. The distribution of Beijing family genotypes differed in different regions. Conclusion The genotypes of Mycobacterium tuberculosis in Beijing are mainly epidemic in Beijing, and are closely related to drug resistance, age and geographical location, and the Beijing family genotypes are mainly monitored.