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目的探讨血管内皮生长因子C(VEGF-C)及其受体3(VEGFR-3)mRNA在上皮性卵巢癌组织的表达及与癌周淋巴管生成及淋巴转移的关系。方法2003-04-2004-06第三军医大学附属西南医院采用RT-PCR方法,检测VEGF-C及VEGFR-3mRNA在良、恶性上皮性卵巢肿瘤组织的表达及组织化学淋巴管染色并计数,分析其与VEGF-CmRNA表达及淋巴转移的关系。结果VEGF-CmRNA和VEGFR-3mRNA在卵巢良、恶性肿瘤的表达差异有显著性。VEGF-CmRNA表达阳性及有淋巴结转移组的癌组织淋巴管密度分别大于VEGF-CmRNA表达阴性及无淋巴结转移组的癌组织,二者差异均有显著性。结论VEGF-CmRNA在上皮性卵巢癌组织的表达上调导致了癌周淋巴管生成,促进了肿瘤的淋巴道转移。
Objective To investigate the expression of vascular endothelial growth factor C (VEGF-C) and its receptor 3 (VEGFR-3) mRNA in epithelial ovarian cancer and its relation with peritumoral lymphangiogenesis and lymphatic metastasis. Methods The expression of VEGF-C and VEGFR-3 mRNA in benign and malignant epithelial ovarian neoplasms and the staining of chemosensitive lymphatic vessels were detected by RT-PCR in the Southwest Hospital affiliated to the Third Military Medical University from 2003-04-2004-06. Its relationship with VEGF-C mRNA expression and lymphatic metastasis. Results The expression of VEGF-C mRNA and VEGFR-3 mRNA in benign and malignant ovarian tumors were significantly different. The lymphatic vessel density of the cancerous tissue with positive VEGF-C mRNA expression and lymph node metastasis was significantly higher than that with negative VEGF-C mRNA expression and no lymph node metastasis, both of which were significantly different. Conclusion The up-regulation of VEGF-C mRNA in epithelial ovarian cancer led to lymphangiogenesis in the pericarcinoma and promoted lymphatic metastasis of the tumor.