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[目的]了解二甲基甲酰胺(DMF)对中国仓鼠肺成纤维细胞(V79细胞)的细胞毒性及DNA损伤作用。[方法]以体外培养V79细胞为研究对象,采用四氮唑盐比色分析法(MTT法)和单细胞凝胶电泳技术(SCGE)分别检测5个浓度(0.5、2.0、8.0、32.0、128.0mmol/L)DMF在3个时间段(6、12、24h)染毒后对V79细胞的毒性作用和DNA损伤情况。[结果]同一时间组V79细胞存活率随染毒浓度的增加而下降,与阴性对照组比较差异均有统计学意义(P<0.05),存在明显的剂量-效应关系(6h:b=-0.002,P<0.05;12h:b=-0.003,P<0.05;24h:b=-0.003,P<0.05)。各染毒浓度组的彗星拖尾率、尾长、Olive尾距、尾部DNA百分含量,与同一时间阴性对照组比较,差异均有统计学意义(P<0.05)。[结论]本实验条件下,DMF能够明显抑制V79细胞增殖,存在正向的剂量-效应关系,并能引起DNA损伤。
[Objective] To investigate the cytotoxicity and DNA damage of dimethylformamide (DMF) on Chinese hamster lung fibroblasts (V79 cells). [Method] V79 cells were cultured in vitro. Five concentrations (0.5,2.0,8.0,32.0,128.0) were detected by MTT assay and single cell gel electrophoresis (SCGE) mmol / L) DMF toxicity to V79 cells and DNA damage after exposure to 3 time periods (6, 12, 24 h). [Results] The survival rate of V79 cells in the same time group decreased with the increase of exposure concentration, and the difference was statistically significant compared with the negative control group (P <0.05), and there was a significant dose-response relationship (6h: b = -0.002 , P <0.05; 12h: b = -0.003, P <0.05; 24h: b = -0.003, P <0.05). The tailing rate, tail length, Olive tail length, percentage of tail DNA in each concentration group were significantly different from those in the negative control group at the same time (P <0.05). [Conclusion] Under the experimental conditions, DMF can significantly inhibit the proliferation of V79 cells, there is a positive dose-response relationship, and can cause DNA damage.