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我们对15例HBsAg阳性(RPHA法)母亲的16例流产死胎开胸、行心脏穿刺采血,并采集了胎肝组织。应用ELISA检测胎儿血清HBsAg及IgM抗HBc,并检测胎儿血清HBV DNA多聚酶活力;免疫电镜法检测胎儿血清及肝匀浆中HBV抗原颗粒;
Sixteen cases of stillbirths from 15 HBsAg positive (RPHA) mothers were thoracotomy, cardiac puncture and blood collection, and fetal liver tissue was collected. HBsAg and IgM anti-HBc were detected by ELISA, and the activity of HBV DNA polymerase in fetal serum was detected. Immunocytochemistry was used to detect HBV antigen particles in fetal serum and liver homogenate.